It's actually an old Python audio sketch adapted to the internate age!
I'm quite certain that that's bullshit. I've come across that animation on several sites (like stileproject.com) and in simple MP3 format on filesharing networks and I still have no idea why it is attributed to the Python's (I've also seen George Carlin credited for it, and it ain't him either). Reasons it ain't Python:
1) It's too stupid, lame and obvious
2) It's obviously not British (and I don't just mean the accent, the whole thing reeks of North America)
5) It's obviously very modern and Python haven't worked together in over two decades (Does "abso-fucking-lutely!" sound very Pythonesque?)
4) It isn't a Python piece. That "joke" has been making its rounds in the form of a list since the advent of the office photocopier. List like
thisMy theory is that, with the new technology and all that...someone decided to make an audio version to be passed around as an MP3 instead of a photocopy and somewhere along the line it came to naming the track, someone slapped the name "Monty Python" on it. There are a couple of reasons why they would do this:
a) to spread it (i.e. if I had done it, having "Matthew Gosse" as the 'artist' would hardly get it circulating)
b) someone entered it in the "style" or "similar artists" option of the MP3
Anyway, eventually someone decided to go a step further and made a flash cartoon out of it (much like that Ebonics Delta Airlines thing Jeff posted) and gave as its source, the mislabelled MP3.
I'm a pretty big Python nut and have pretty much seen it all, heard it all, read it all, own it all and I've never heard of sketch like this.
I suppose there is the off chance that this is a modernization of a something they did, but that audio definitely has nothing to do with Python.